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Chinese Journal of Brain Diseases and Rehabilitation(Electronic Edition) ›› 2018, Vol. 08 ›› Issue (01): 37-40. doi: 10.3877/cma.j.issn.2095-123X.2018.01.009

Special Issue:

• Basic Research • Previous Articles     Next Articles

Relationship between ClC-3, BK type of ion channel and the invasive growth of glioma

Peigang Lu1,(), Yuan Dong1, Bo Li1, Kuizhong Wang1, Zhenqiang Hao1, Guanzhong Qiu1, Jingzheng Cao2   

  1. 1. Department of Neurosurgery, General Hospital of Ji’nan Military Command, Ji’nan 250031, China
    2. Department of Neurosurgery, People’s Hospital of Jining, Jining 272011, China
  • Received:2018-11-09 Online:2018-12-15 Published:2018-12-15
  • Contact: Peigang Lu
  • About author:
    Corresponding author: Lu Peigang, Email:

Abstract:

Objective

To observe the expression of ClC-3 and BK ion channels in rat glioma cells, and speculate the function of ClC-3 and BK in the invasion of glioma cells.

Methods

Conventional establishment of C6 brain glioma model in Wistar rats, Wistar rats were randomly divided into 5 groups (glioma model group, glioma model+Chlorotoxin group, glioma model+Iberiotoxin group, sham operation group and control group). The immunohistochemical detection of ClC-3 and BK expression in glioma rat model was performed. Furthermore, the changes of tumor volume after using CIC-3 specific inhibitor Chlorotoxin and BK inhibitor Iberiotoxin were observed.

Results

ClC-3 ion channel was highly expressed in the C6 glioma cell line, while the BK ion channel expression was relatively low; the tumor volum of C6 brain glioma rats decreased significantly after application of Chlorotoxin (P<0.05), while there was no significant change in tumor volum of C6 brain glioma rats after the application of Iberiotoxin.

Conclusion

ClC-3 ion channel plays an important role in the invasive growth of glioma cells, suggesting that ClC-3 ion channels might be a new target for glioma treatment in the future.

Key words: Glioma, Invasion, ClC-3 ion channel, BK ion channel

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