Study of the expression of miRNA-874 in glioblastoma stem cells based on microfluidic platform

doi:10.3877/cma.j.issn.2095-123X.2020.04.003

Abstract

Abstract:

Objective

To investigate the role and significance of the expression of miRNA-874 (miR-874) in the growth of glioblastoma stem cells (GSCs) by microfluidic detection platform.

Methods

Using high-throughput, multi-sample and low-cost microfluidic technology, GSCs and neural stem cells (NSCs) were transfected with miR-874. A series of cell molecular biological experiments, such as qPCR detection of intracellular miR-874 expression, were carried out to study the effect of miR-874 on the growth and apoptosis of glioblastoma stem cells.

Results

The expression of miR-874 in GSCs was significantly decreased, about 2%-40% of the expression in NSCs. By transfection of miR-874 in GSCs with dendrimer, the number of GSCs decreased significantly and the numb indicating a killing effect of the GSCs by the miR-874.

Conclusion

The expression of miR-874 decreased significantly in GSCs. Overexpression of miR-874 in GSCs leads to significant cell growth inhibition and death, which is helpful for the development of novel brain cancer drugs and therapies based on miRNA.

Key words: Glioblastoma stem cell, Neural stem cell, Microfluidic platform, miRNA-874

Yanzhao Li, Xuxin Zhang, Jing Sun, Mingfei Lang, Gang Ren, Dongfeng Deng. Study of the expression of miRNA-874 in glioblastoma stem cells based on microfluidic platform[J]. Chinese Journal of Brain Diseases and Rehabilitation(Electronic Edition), 2020, 10(04): 205-208.

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References 16

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