To explore the protective effect of cornel iridoid glycoside (CIG) on 6-hydroxydopa (6-OHDA)-induced damage to Parkinson's disease (PD) cell model.

PC12 cells were divided into blank control group (untreated), PD model group (with 6-OHDA induced PD cell model), PD model+CIG 20 μmol/L group (with 6-OHDA and 20 μmol/L CIG), PD model+CIG 40 μmol/L group (administered 6-OHDA and 40 μmol/L CIG), PD model+CIG 80 μmol/L group (administered 6-OHDA and 80 μmol/L CIG). Cell counting kit 8 method was used to detect cell survival, DCFH-DA fluorescent probe was applied to assess ROS content, flow cytometry was performed to determine cell apoptosis, and colorimetric analysis of superoxide dismutase (SOD) activity and malondialdehyde (MDA) content, enzyme-linked immunosorbent assay to investigate the secretion of inflammatory factors tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), and Western blot to determine Bcl-2, Bax, Caspase3, Cleaved Caspase3, P47phox, TH, HO-1, Nrf2 and NQO1 protein expression.

Compared with the blank control group, cell viability, SOD activity, Bcl-2, TH, HO-1, Nrf2 and NQO1 protein expression levels of PC12 cells in the PD model group were significantly reduced, and the apoptosis rate, ROS content, MDA content, Bax, Caspase3, Cleaved Caspase3, P47phox protein expression levels, TNF-α, IL-1β content were significantly increased (P<0.05). Compared with the PD model group, the PD model+CIG 20 μmol/L group, PD model+CIG 40 μmol/L group, PD model+CIG 80 μmol/L group PC12 cell viability, SOD Activity, Bcl-2, TH, HO-1, Nrf2 and NQO1 protein expression levels were remarkably increased, apoptosis rate, ROS content, MDA content, Bax, Caspase3, Cleaved Caspase3, P47phox protein expression levels, TNF-α, IL-1β content obviously reduced, all showed concentration dependence (P<0.05).

CIG can promote 6-OHDA-induced PC12 cell proliferation, inhibit apoptosis, reduce oxidative stress damage and improve inflammatory response, thereby protecting the PD cell model damage.