Taking supraorbital keyhole approach operation to build cynomolgus monkey middle cerebral artery occlusion model

doi:10.3877/cma.j.issn.2095-123X.2024.01.002

Abstract

Abstract:

Objective

To evaluate the effectiveness of building cynomolgus monkey middle cerebral artery occlusion (MCAO) model by taking supraorbital keyhole approach operation.

Methods

Four ordinary grade experimental monkeys were randomly divided into two groups. Three experimental monkeys of operation group underwent left supraorbital keyhole approach craniotomy, initial part of M1 segment of left middle cerebral artery was exposed and then coagulated and cut to make MCAO model. One experimental monkey of control group underwent left supraorbital keyhole approach craniotomy, initial part of M1 segment of left middle cerebral artery was exposed but not coagulated and cut to make negative control. All experimental monkeys were evaluated using the non-human primate stroke scale (NHPSS) at 24 h, 3 d, 7 d, 14 d, and 28 d postoperatively. Brain MRI axial T2 weighted scans were performed on all experimental monkeys at 7 d postoperatively.

Results

All experimental monkeys survived after operation without any significant postoperative complications, and lived well during experimental period. The NHPSS scores of the operation group at postoperative time of 24 h, 3 d, 7 d, 14 d, 28 d was (19.67±1.15), (22.00±1.00), (19.33±1.15), (18.67±1.53), (18.00±1.00) points respectively. The NHPSS scores of the control group at postoperative time of 24 h, 3 d, 7 d, 14 d, 28 d were all 0 points. Brain MRI axial T2 weighted scan at 7 d after operation showed that brain infarction focus appeared in the blood supply area of left middle cerebral artery of all experimental monkeys of operation group, scattered in the left frontal lobe, parietal lobe, temporal lobe and basal ganglion area, and accompanied with mild cerebral edema, whereas no brain infarction focus and brain edema was appeared in the experimental monkey of control group.

Conclusion

The application of the supraorbital keyhole approach surgical method has the advantage of minimally invasion, reliable model effect, good repeatability. The approach method has good repeatability and can make mass production of cynomolgus monkey MCAO models.

Key words: Middle cerebral artery occlusion, Supraorbital, Keyhole approach, Cynomolgus monkey, Animal model

Chenyu Chu, Qiang Xu, Junhua Rao, Yuefeng Li. Taking supraorbital keyhole approach operation to build cynomolgus monkey middle cerebral artery occlusion model[J]. Chinese Journal of Brain Diseases and Rehabilitation(Electronic Edition), 2024, 14(01): 8-13.

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References 20

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