A study on the relation of positive and negative symptoms to long non-coding RNAs with altered expressions in schizophrenia

doi:10.3877/cma.j.issn.2095-123X.2017.02.004

Abstract

Abstract:

Objective

To explore the relation of positive and negative symptoms to long non coding RNAs (lncRNAs) with altered expressions in peripheral blood of schizophrenia.

Methods

Gene microarray was used to screen the lncRNAs which expressed differently in case group compared to that in control group, and the screened lncRNAs were validated by real-time fluorescence quantitative PCR(qRT-PCR) in 96 schizophrenic patients in the outpatient service and psychiatric wards of No.102 Hospital of the Chinese People′s Liberation Army and 44 healthy people from Auguest 2012 to June 2014. And Mann-Whitney U test, t test and correlation analysis were used to analyze the correlations of positive and negative symptoms with the expression levels of lncRNAs in schizophrenic patients.

Results

1. Compared to control group, 3 lncRNAs(NONHSAT021545、NONHSAT089447、NONHSAT098126) in case group were greatly high-regulated and the differences were statistically significant (t=-2.460~-2.038, P<0.05). 2. The correlation analysis on differentially-expressed lncRNAs with the scores of positive and negative symptom scale (PANSS) revealed that PR1~PR10 with altered expressions were positively associated with the scores of positive and negative symptom scale (r=0.231~0.276, all P<0.05). 3. PR1~PR10 with altered expressions were positively correlated to activation(r=0.243~0.352, all P<0.05); TCONS_l2_00025502、ENST00000563823、ENST00000521622、TCONS_l2_00021339 and NONHSAT104778 were positively correlated to stubborn(r=0.201~0.223, P<0.05); PR1~PR10 were positively correlated to aggressivity(r=0.207~0.259, P<0.05). 4. The scores of activation and aggressivity of NONHSAT021545 and NONHSAT041499 in high-expressed group were significantly lower than that in low-expressed group(P<0.05 or 0.01).

Conclusion

The abnormal expression of lncRNAs may have some relevance with schizophrenia, and to be specific, some lncRNAs may be associated with the positive and negative symptoms.

Key words: Schizophrenia, Long non-coding RNA, Biomarker, Positive and negative symptom scale

Mingjun He, Gaofeng Yao, Liyi Zhang, Lingming Kong, Wei Niu, Shendong Chen, Aifang Zhong. A study on the relation of positive and negative symptoms to long non-coding RNAs with altered expressions in schizophrenia[J]. Chinese Journal of Brain Diseases and Rehabilitation(Electronic Edition), 2017, 07(02): 64-70.

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References 30

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TargetID	FC (abs)	Regulation	P
NONHSAT021545	3.697919	up	0.023714
NONHSAT089447	3.514152	up	0.038920
NONHSAT098126	4.610542	up	0.047744

TargetID	FC (abs)	Regulation	P
NONHSAT021545	3.697919	up	0.023714
NONHSAT089447	3.514152	up	0.038920
NONHSAT098126	4.610542	up	0.047744

lncRNA	例数	PR1	PR2	PR3	PR4	PR5	PR6	PR7	PR8	PR9	PR10
病例组	96	4.14±4.28	5.03±4.72	5.21±4.95	4.23±4.79	3.60±4.57	4.51±4.56	5.05±3.98	5.80±4.82	3.75±4.87	5.67±4.80
对照组	44	5.73±4.43	6.50±5.11	6.83±5.38	6.28±5.37	5.31±4.65	6.48±4.50	6.70±4.45	7.50±5.33	5.55±5.33	4.76±5.10
Z值	?	－2.042	－1.571	－1.688	－2.038	－1.921	－2.177	－2.460	－1.742	－1.836	－1.737
P值	?	0.041	0.116	0.091	0.042	0.055	0.029	0.014	0.082	0.066	0.082

lncRNA	例数	PR1	PR2	PR3	PR4	PR5	PR6	PR7	PR8	PR9	PR10
病例组	96	4.14±4.28	5.03±4.72	5.21±4.95	4.23±4.79	3.60±4.57	4.51±4.56	5.05±3.98	5.80±4.82	3.75±4.87	5.67±4.80
对照组	44	5.73±4.43	6.50±5.11	6.83±5.38	6.28±5.37	5.31±4.65	6.48±4.50	6.70±4.45	7.50±5.33	5.55±5.33	4.76±5.10
Z值	?	－2.042	－1.571	－1.688	－2.038	－1.921	－2.177	－2.460	－1.742	－1.836	－1.737
P值	?	0.041	0.116	0.091	0.042	0.055	0.029	0.014	0.082	0.066	0.082

lncRNA	阳性量表	阴性量表	一般病理量表	复合量表
ENST00000394742	0.244^a	－0.004	－0.006	0.000
TCONS_l2_00025502	0.276^b	－0.019	－0.023	0.014
NONHSAT098126	0.252^a	0.012	－0.026	－0.006
NONHSAT089447	0.258^a	－0.009	－0.018	0.030
ENST00000563823	0.257^a	0.011	－0.007	0.030
NONHSAT021545	0.249^a	－0.025	－0.027	0.008
NONHSAT041499	0.231^a	0.002	0.011	－0.041
ENST00000521622	0.247^a	0.007	0.020	0.028
TCONS_l2_00021339	0.260^a	0.009	0.002	0.055
NONHSAT104778	0.247^a	－0.007	－0.006	0.021

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