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中华脑科疾病与康复杂志(电子版) ›› 2019, Vol. 09 ›› Issue (03) : 132 -136. doi: 10.3877/cma.j.issn.2095-123X.2019.03.002

所属专题： 文献；

临床研究

脑脊液miRNA-22水平对儿童细菌性脑膜炎的早期诊断及疗效分析
林碧惠1,†(), 简咏芬2, 蔡艳1, 范徐威1, 杨燕华1, 王翠敏1   
  1. 1. 528200　佛山市南海区妇幼保健院儿内科
    2. 528200　佛山市第二人民医院检验科
  • 收稿日期:2019-04-11 出版日期:2019-06-15
  • 通信作者: 林碧惠
  • 基金资助:
    佛山市医学类科技攻关项目(2016AB000782)

Clinical study of miRNA-22 level in cerebrospinal fluid using in bacterial meningitis early diagnosing and prediction of therapeutic effect

Bihui Lin1,†(), Yongfen Jian2, Yan Cai1, Xuwei Fan1, Yanhua Yang1, Cuimin Wang1   

  1. 1. Department of Pediatric Internal Medicine, The Maternal and Child Care Service Center of Nanhai District, Foshan 528200, China
    2. Department of Clinical Laboratory, The Second People’s Hospital of Foshan City, Foshan 528200, China
  • Received:2019-04-11 Published:2019-06-15
  • Corresponding author: Bihui Lin
  • About author:
    Corresponding author: Lin Bihui, Email:
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林碧惠, 简咏芬, 蔡艳, 范徐威, 杨燕华, 王翠敏. 脑脊液miRNA-22水平对儿童细菌性脑膜炎的早期诊断及疗效分析[J]. 中华脑科疾病与康复杂志(电子版), 2019, 09(03): 132-136.

Bihui Lin, Yongfen Jian, Yan Cai, Xuwei Fan, Yanhua Yang, Cuimin Wang. Clinical study of miRNA-22 level in cerebrospinal fluid using in bacterial meningitis early diagnosing and prediction of therapeutic effect[J]. Chinese Journal of Brain Diseases and Rehabilitation(Electronic Edition), 2019, 09(03): 132-136.

目的

研究脑脊液miRNA-22用于早期诊断儿童化脓性脑膜炎及其治疗效果预测的临床价值。

方法

选取佛山市南海区妇幼保健院自2015年1月至2018年3月收治的82例脑膜炎患儿和佛山市第二人民医院同期收治的44例脑膜炎患儿，根据确诊病因类型分为细菌性脑膜炎组（n=64）、病毒性脑膜炎组（n=44）、结核性脑膜炎组（n=18）。比较3组患儿治疗前和治疗后脑脊液miRNA-22拷贝数差异。常规标准治疗下将细菌性脑膜炎患儿治疗效果情况分为满意（28例）、一般（19例）、欠佳（17例），比较不同治疗效果下在入院时、治疗后第1、3、5天时脑脊液miRNA-22水平差异以评价其水平、变化趋势以及与疾病治疗效果的关系。

结果

相较于病毒性脑膜炎组和结核性脑膜炎组，细菌性脑膜炎组治疗前miRNA-22的拷贝数明显更高，差异具有统计学意义（P<0.05）。细菌性脑膜炎组经过治疗后，脑脊液miRNA-22的水平呈现快速下降的趋势，差异具有统计学意义（P<0.05）。细菌性脑膜炎组患儿入院时脑脊液miRNA-22水平差异无统计学意义（P>0.05），但疗效越差患儿入院时miRNA的水平更高；治疗效果满意的细菌性脑膜炎患儿的脑脊液miRNA-22拷贝数明显较疗效一般和欠佳的患儿更低，差异具有统计学意义（P<0.05）。

结论

脑膜炎患儿脑脊液中miRNA-22拷贝数的水平对早期区分脑膜炎病原菌的类型有一定的参考价值，细菌性脑膜炎患儿脑脊液中的miRNA-22拷贝数明显升高，且miRNA-22拷贝数与细菌性脑膜炎患儿的预期治疗效果有关。

关键词: miRNA-22, 细菌性脑膜炎, 早期诊断, 儿童
Objective

To research the clinical value of miRNA-22 level in cerebrospinal fluid (CSF) in bacterial meningitis early diagnosing and prediction of therapeutic effect.

Methods

Eighty-two children with meningitis admitted to The Maternal and Child Care Service Center of Nanhai District from January 2015 to March 2018 and 44 children with meningitis admitted to the Second People’s Hospital of Foshan City at the same time were selected. They were divided into bacterial meningitis group (n=64), viral meningitis group (n=44), tuberculous meningitis group (n=18) according to the type of etiology. The copies of miRNA-22 in CSF of three groups were compared before and after treatment. The therapeutic effect of bacterial meningitis in children was divided into satisfying group (28 cases), general group (19 cases) and indisposed group (17 cases) under the conventional standard treatment. The levels of miRNA-22 in CSF were compared before and after treatment at 1, 3 and 5 d in order to evaluate the relationship between the level and variation tendency of miRNA-22 and the therapeutic effect of the disease.

Results

Compared with viral meningitis group and tubercular meningitis group, the copy number of miRNA-22 in bacterial meningitis group is significantly higher before treatment (P<0.05). After standard treatment, the copy number of miRNA-22 in CSF of bacterial meningitis group is declining quickly and significantly than the other groups (P<0.05). There was no significant difference in the level of miRNA-22 in CSF of bacterial meningitis group at admission (P>0.05), but the worse the curative effect, the higher the level of miRNA-22 at admission. the copy number of miRNA-22 in CSF of bacterial meningitis group with satisfactory therapeutic effect was significantly lower than general group and indisposed group.

Conclusion

The level of miRNA-22 copies in CSF of children with meningitis has certain reference value for early distinguishing the types of pathogenic bacteria in children with meningitis. The number of miRNA-22 copies in CSF of children with bacterial meningitis is significantly increased, and the number of miRNA-22 copies may be correlated with the anticipated therapeutic effect of children with bacterial meningitis.

Key words: miRNA-22, Bacterial meningitis, Early diagnosing, Children
图1 不同病因组脑膜炎患儿脑脊液水平miRNA-22在不同时间点的拷贝数比较
图2 细菌性脑膜炎患儿不同疗效组间miRNA-22水平比较
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