Molecular mechanism of verbascoside in the treatment of glioblastoma by inhibiting STAT3 phosphorylation by upregulating SHP-1 expression

doi:10.3877/cma.j.issn.2095-123X.2021.05.007

Abstract

Abstract:

Objective

To confirm the effects of verbascoside (VB) on glioblastoma and explore the potential molecular mechanism.

Methods

The Thiazolyl blue assay was used to test cell proliferation, flow cytometry was used to detect cell apoptosis, and a transwell assay was used for cell migration and invasion. The tumor formation experiment in nude mice was used to verify the tumor inhibition ability of VB in vivo. Western blot analysis was employed to determine the protein expression of related genes.

Results

After VB treatment, the proliferation, migration and invasion of glioblastoma cells decreased, and apoptosis increased. Western blot showed that the expression of related signal pathway Src-homology domain 2-containing protein tyrosine phosphatase 1 (SHP-1) increased, the expression of phosphorylated signal transduction and transcriptional activator (STAT3) down-regulated, the expression of downstream related Pro apoptotic genes increased, and the expression of genes that inhibit apoptotic genes, promote cell proliferation, survival and migration decreased. In nude mice transplanted tumor model, compared with the control group, VB treatment could reduce the tumor volume (P<0.05). At the same time, VB can enhance the anti-glioblastoma effect of temozolomide, a traditional chemotherapeutic drug.

Conclusion

VB can inhibit the phosphorylation of STAT3 by up regulating the expression of SHP-1, and then produce the effect of anti-glioblastoma

Key words: Glioblastoma, Verbascoside, Tyrosine phosphatase 1, Phosphorylated signal transduction and transcriptional activator, Migration, Invasion, Apoptosis

Weiqiang Jia, Zhaotao Wang, Ruxiang Xu. Molecular mechanism of verbascoside in the treatment of glioblastoma by inhibiting STAT3 phosphorylation by upregulating SHP-1 expression[J]. Chinese Journal of Brain Diseases and Rehabilitation(Electronic Edition), 2021, 11(05): 285-293.

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Figures/Tables 7

References 23

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