To compare the characteristics of sleep structure in patients with epilepsy at different times.

From March 2016 to December 2016, 88 patients with epilepsy in the Department of Neurology, the First Hospital of Shanxi Medical University were enrolled in this study, eighty patients with age and gender matched with epilepsy were selected as the subjects. According to the different seizures of epilepsy, epilepsy patients were divided into three groups: the night attack group (30 cases of epilepsy only at night), daytime attack group (30 cases of epilepsy only during daytime), and mixed attack group (28 cases of epilepsy patients who had seizures during the day and night), and healthy people (80 cases) as the control group. All groups were given polysomnography (PSG) in neurological electroencephalograph (EEG) room. The sleep parameters were analyzed by polysomnography, and the sleep structure of the epilepsy group and the healthy control group were compared. The sleep structure of the three groups of epilepsy patients was further compared.

1. Comparison of epilepsy group and healthy control group: the total sleep time was significantly shortened (P<0.05), sleep efficiency was significantly decreased (P<0.05), sleep latency, rapid eye movement sleep (REM) latency were extended (P<0.05). The percentage of total sleep time was significantly increased in non-rapid eye movement sleep (NREM) stage 1 and 2 and awake period (all P<0.05), NREM3 + 4 period, REM period accounted for the total sleep time percentage was significantly reduced (P<0.05). 2.Night attack group compared with daytime attack group: total sleep time was significantly reduced(P<0.05), sleep latency and REM latency prolonged, sleep efficiency decreased significantly, NREM1 and 2 stage and awake period significantly increased the percentage of total sleep time, NREM3 + 4 period, REM period accounted for the total sleep time percentage was significantly reduced (P<0.05). 3.Night attack group compared with mixed attack group: sleep latency and REM latency were significantly prolonged (P<0.05), NREM1 and 2 stage and awake accounted for a significant increase in the percentage of total sleep time (P<0.05), while the total sleep time was significantly reduced, sleep efficiency decreased significantly, NREM 3+ 4 period and REM period decreased the total sleep time percentage.4.Comparison of the mixed attack group and the daytime attack group: the total sleep time was significantly reduced(P<0.05), sleep efficiency decreased significantly(P<0.05), NREM1 and 2 stage and awake period increased the percentage of total sleep time(P<0.05), NREM3 + 4 and REM decreased the percentage of total sleep time(P<0.05), while the sleep latency and REM latency were significantly prolonged(P<0.05).

1.There is a significant difference in the sleep structure between the epilepsy group and the healthy control group.2.There are significant differences in sleep structure among the three groups of night group, daytime attack group and mixed attack group, and the sleep structure of night attack group changes more.

To observe the improvment on patients who has dysphagia after stroke after strengthening the training of respiratory function and normal swallowing function.

Use Random digital table to devide 60 patients into two groups-control group and treatment group. And all patients used to be treated in the Department of Rehabilitation between June 2014 and June 2016. Ther were 30 cases in each group. The control group were treated with conventional swallowing function training with low frequency electrical stimulation, swallowing training was divided into direct and indirect training. Including exercise training of swallowing organs, thermal stimulation training, swallowing auxiliary techniques and the adjustment of viscosity. On the basis of control group′s training, treatment group strengthen the training of respiratory function, which 2 times a day, once a 10 minutes. Both two groups did the test before the treatment and eight weeks after treatment. We use water swallow test, the standard of assessing degree of stroke neurological impairment on dysphagia subscale Rosenbek aspiration level grading to evaluate the efficacy.

Before treatment, these two groups barely had difference on water swallow test, the standard of assessing degree of stroke neurological impairment on dysphagia subscale, Rosenbek aspiration level grading(P>0.05), while after 8 week′s treatment, patients in treatment group improved a lot on water swallow test, the standard of assessing degree of stroke neurological impairment on dysphagia subscale, Rosenbek aspiration level grading(P<0.05). Treatment group′s scores were [( 2.03±0.68), (8.52±1.66) and (2.55±1.12)] compared with the control group [(2.55±0.91), (7.21±2.29) and (3.55±1.82)]. It has significantly improvement and has statistically significant (P<0.05)

Strengthening the training of respiratory function and normal swallowing function both can effectively improve the swallowing function in patients with stroke, and if increase training in respiratory function gonna be better.

To explore the relation of positive and negative symptoms to long non coding RNAs (lncRNAs) with altered expressions in peripheral blood of schizophrenia.

Gene microarray was used to screen the lncRNAs which expressed differently in case group compared to that in control group, and the screened lncRNAs were validated by real-time fluorescence quantitative PCR(qRT-PCR) in 96 schizophrenic patients in the outpatient service and psychiatric wards of No.102 Hospital of the Chinese People′s Liberation Army and 44 healthy people from Auguest 2012 to June 2014. And Mann-Whitney U test, t test and correlation analysis were used to analyze the correlations of positive and negative symptoms with the expression levels of lncRNAs in schizophrenic patients.

1. Compared to control group, 3 lncRNAs(NONHSAT021545、NONHSAT089447、NONHSAT098126) in case group were greatly high-regulated and the differences were statistically significant (t=-2.460~-2.038, P<0.05). 2. The correlation analysis on differentially-expressed lncRNAs with the scores of positive and negative symptom scale (PANSS) revealed that PR1~PR10 with altered expressions were positively associated with the scores of positive and negative symptom scale (r=0.231~0.276, all P<0.05). 3. PR1~PR10 with altered expressions were positively correlated to activation(r=0.243~0.352, all P<0.05); TCONS_l2_00025502、ENST00000563823、ENST00000521622、TCONS_l2_00021339 and NONHSAT104778 were positively correlated to stubborn(r=0.201~0.223, P<0.05); PR1~PR10 were positively correlated to aggressivity(r=0.207~0.259, P<0.05). 4. The scores of activation and aggressivity of NONHSAT021545 and NONHSAT041499 in high-expressed group were significantly lower than that in low-expressed group(P<0.05 or 0.01).

The abnormal expression of lncRNAs may have some relevance with schizophrenia, and to be specific, some lncRNAs may be associated with the positive and negative symptoms.

To investigate the psychological characteristics of patients with refractory obsessive-compulsive disorder in employment or unemployment.

Ninty eight patients with refractory obsessive-compulsive disorder from May 2009 to October 2015 were selected into the study. The patients were divided into the employed group(56 cases) and the unemployed group (42 cases). The evaluation of egna minnen barndoms uppfostran (EMBU) and defense style questionnaire (DSQ) of the two groups were compared. All the data were analyzed by SPSS 12.0.

In the way of parenting, the warmth factor of the employed group was higher than that of unemployed group, the two groups have significant differences (P<0.05). In psychological defense mechanisms scale, the evading factor of the employed group was lower than that of unemployed group, the two groups have significant differences (P<0.05).

Unemployment puts more pressure on patients, that will harm to rehabilitation, and the psychological characteristics also influence emplyment of the patients with refractory obsessive-compulsive disorder. Improve the professional competence of patients with obsessive-compulsive disorder is an important aspect of the patients return to society.

To investigate the expression of serum protein S100β in patients with isolated vertigo, analyze the diagnosis effect on acute posterior circulation stroke and nonvascular vertigo, which provide the evidence for early diagnosis and treatment of isolated vertigo.

The patients with acute isolated vertigo were divided into two groups, the stroke group (50 cases) and the nonvascular vertigo group (50 cases), and the empty serum protein S100β expression levels in 24 hours was tested .

The levels of serum protein S100β in the stroke group were significantly higher than the nonvascular vertigo group, with a statistical difference (t=6.456, P=0.000 ).

S100β could serve as a biomarker for distinguishing between acute posterior circulation stroke and nonvascular vertigo.

To investigate the protective effect of limb remote ischemic postconditioning (RIPostC) on vascular cognitive impairment (VCI).

According to random number table, twelve healthy male C57BL / 6J mice were randomly divided into 3 groups: sham operation group (sham group, n=4), model control group (BCAS group, n= 4) and model-distal ischemia group (BCAS + RIPostC group, n=4). The bilateral carotid artery stenosis (BCAS) was used to produce mice model. Two weeks after the BCAS surgery, BCAS + RIPostC mice were treated with RIPostC at the same time for 5 minutes, repeated 4 times a day for 2 weeks. And morris water maze test was used to test the cognitive function. HE staining was used to detect pathological changes of white matter in each group at the same time. The expression of Beclin-1 protein in the white matter was detected by Western blot.The repeated measurement ANOVA was used to analyse verage escape latency of mice. The remaining data were analyzed by one-way ANOVA, LSD-t test was used to compare two groups.

Twenty eight days later , The water maze test showed that the BCAS group and the BCAS + RIPostC group had a longer escape latency than the BCAS group(P>0.05). Compared with the sham group, The mean escape latency of RIPostC group was shortened(P>0.05). HE staining showed that RIPostC could improve the degree of white matter damage induced by BCAS. Western blot showed that the expression of Beclin-1 in BCAS group and BCAS + RIPostC group was significantly higher than that in sham group (P <0.05), and the expression of Beclin-1 in BCAS + RIPostC group increased more significantly (P <0.05).

RIPostC can reduce the damage of brain white matter caused by BCAS, and the neuroprotective effect of RIPostC may be related to the autophagy pathway.