切换至 "中华医学电子期刊资源库"

中华脑科疾病与康复杂志(电子版) ›› 2020, Vol. 10 ›› Issue (04) : 205 -208. doi: 10.3877/cma.j.issn.2095-123X.2020.04.003

所属专题: 文献

颅内肿瘤

基于微流控平台的胶质母细胞瘤干细胞中miRNA-874表达的研究
李彦钊1, 张绪新1, 孙晶1, 郎明非1, 任刚1, 邓东风1,()   
  1. 1. 116001 大连,大连大学附属中山医院神经外科
  • 收稿日期:2020-07-21 出版日期:2020-08-15
  • 通信作者: 邓东风

Study of the expression of miRNA-874 in glioblastoma stem cells based on microfluidic platform

Yanzhao Li1, Xuxin Zhang1, Jing Sun1, Mingfei Lang1, Gang Ren1, Dongfeng Deng1,()   

  1. 1. Department of Neurosurgery, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China
  • Received:2020-07-21 Published:2020-08-15
  • Corresponding author: Dongfeng Deng
引用本文:

李彦钊, 张绪新, 孙晶, 郎明非, 任刚, 邓东风. 基于微流控平台的胶质母细胞瘤干细胞中miRNA-874表达的研究[J]. 中华脑科疾病与康复杂志(电子版), 2020, 10(04): 205-208.

Yanzhao Li, Xuxin Zhang, Jing Sun, Mingfei Lang, Gang Ren, Dongfeng Deng. Study of the expression of miRNA-874 in glioblastoma stem cells based on microfluidic platform[J]. Chinese Journal of Brain Diseases and Rehabilitation(Electronic Edition), 2020, 10(04): 205-208.

目的

通过微流控检测平台,研究miRNA-874(miR-874)表达在胶质母细胞瘤干细胞(GSCs)生长中的作用及意义。

方法

应用高通量、多样本、低成本的微流控技术,对GSCs和神经干细胞(NSCs)进行miR-874转染,并通过荧光定量PCR检测细胞内miR-874和mRNA表达等一系列细胞分子生物实验,研究miRNA-874对GSCs生长和凋亡的作用。

结果

miR-874在GSCs中的表达显著降低,约为NSCs中表达量的2%~40%(P<0.05)。利用树状分子将miR-874转染入GSCs中后,GSCs的细胞数量显著下降,凋亡细胞的数量显著上升,提示miR-874对GSCs具有杀伤作用。

结论

miR-874在GSCs中表达显著降低,在GSCs中过表达miR-874会导致显著的细胞生长抑制和死亡,有助于开发基于miRNA的新型脑癌药物和疗法。

Objective

To investigate the role and significance of the expression of miRNA-874 (miR-874) in the growth of glioblastoma stem cells (GSCs) by microfluidic detection platform.

Methods

Using high-throughput, multi-sample and low-cost microfluidic technology, GSCs and neural stem cells (NSCs) were transfected with miR-874. A series of cell molecular biological experiments, such as qPCR detection of intracellular miR-874 expression, were carried out to study the effect of miR-874 on the growth and apoptosis of glioblastoma stem cells.

Results

The expression of miR-874 in GSCs was significantly decreased, about 2%-40% of the expression in NSCs. By transfection of miR-874 in GSCs with dendrimer, the number of GSCs decreased significantly and the numb indicating a killing effect of the GSCs by the miR-874.

Conclusion

The expression of miR-874 decreased significantly in GSCs. Overexpression of miR-874 in GSCs leads to significant cell growth inhibition and death, which is helpful for the development of novel brain cancer drugs and therapies based on miRNA.

图1 胶质母细胞瘤干细胞鉴定(×400)
图2 微流控芯片构造示意图
图3 miRNA-874在神经干细胞及胶质母细胞瘤干细胞中的表达
图4 miRNA-874过表达对胶质母细胞瘤干细胞的影响
[1]
Walsh KM, Wiencke JK, Lachance DH, et al. Telomere maintenance and the etiology of adult glioma[J]. Neuro Oncol, 2015, 17(11): 1445-1452.
[2]
Ostrom QT, Gittleman H, Liao P, et al. Cbtrus statistical report: primary brain and central nervous system tumors diagnosed in the United States in 2007-2011[J]. Neuro Oncol, 2014, 16 Suppl 4(Suppl 4): iv1-iv63.
[3]
Chow DS, Horenstein CI, Canoll P, et al. Glioblastoma induces vascular dysregulation in nonenhancing peritumoral regions in humans[J]. AJR Am J Roentgenol, 2016, 206(5): 1073-1081.
[4]
Wen PY, Kesari S. Malignant gliomas in adults[J]. N Engl J Med, 2008, 359(5): 492-507.
[5]
Wicha MS, Liu S, Dontu G. Cancer stem cells: an old idea--a paradigm shift[J]. Cancer Res, 2006, 66(4): 1883-1890; discussion 1895-1886.
[6]
Bao S, Wu Q, McLendon RE, et al. Glioma stem cells promote radioresistance by preferential activation of the DNA damage response[J]. Nature, 2006, 444(7120): 756-760.
[7]
Fallahi-Sichani M, Honarnejad S, Heiser LM, et al. Metrics other than potency reveal systematic variation in responses to cancer drugs[J]. Nat Chem Biol, 2013, 9(11): 708-714.
[8]
Carrasco-Garcia E, Sampron N, Aldaz P, et al. Therapeutic strategies targeting glioblastoma stem cells[J]. Recent Pat Anticancer Drug Discov, 2013, 8(3): 216-227.
[9]
White AC, Lowry WE. Refining the role for adult stem cells as cancer cells of origin[J]. Trends Cell Biol, 2015, 25(1): 11-20.
[10]
Liu HK, Wang Y, Belz T, et al. The nuclear receptor tailless induces long-term neural stem cell expansion and brain tumor initiation[J]. Genes Dev, 2010, 24(7): 683-695.
[11]
Lang MF, Yang S, Zhao C, et al. Genome-wide profiling identified a set of miRNAs that are differentially expressed in glioblastoma stem cells and normal neural stem cells[J]. PLoS One, 2012, 7(4): e36248.
[12]
Floyd DH, Zhang Y, Dey BK, et al. Novel anti-apoptotic microRNAs 582-5p and 363 promote human glioblastoma stem cell survival via direct inhibition of caspase 3, caspase 9, and Bim[J]. PLoS One, 2014, 9(5): e96239.
[13]
杜春发,王琼,张飚,等.微小RNA-874对U87胶质瘤细胞增殖和凋亡的影响[J].中华实验外科杂志, 2015, 32(3): 525-527.
[14]
Taylor RJ, Falconnet D, Niemisto A, et al. Dynamic analysis of MAPK signaling using a high-throughput microfluidic single-cell imaging platform[J]. Proc Natl Acad Sci USA, 2009, 106(10): 3758-3763.
[15]
Wu HW, Lin CC, Lee GB. Stem cells in microfluidics[J]. Biomicrofluidics, 2011, 5(1): 013401.
[16]
Zhang Q, Austin RH. Applications of microfluidics in stem cell biology[J]. Bionanoscience, 2012, 2(4): 277-286.
[1] 周伟, 蔡恒, 范海迪, 李惠中, 王传霞, 顾茂胜. cblC型甲基丙二酸血症MMACHC基因新突变对小鼠神经细胞凋亡及Wnt/β-catenin信号通路的作用机制[J]. 中华妇幼临床医学杂志(电子版), 2022, 18(05): 528-539.
[2] 刘伟华, 赵宇, 刘仲凤, 吴焕童, 张广吉, 陈志国. 神经干细胞生物制剂治疗中枢神经系统恶性肿瘤的研究进展[J]. 中华细胞与干细胞杂志(电子版), 2022, 12(01): 59-62.
[3] 王泽宁, 侯博儒, 姜呈, 任海军. 小胶质细胞对神经干细胞调控机制的研究进展[J]. 中华细胞与干细胞杂志(电子版), 2020, 10(03): 172-176.
[4] 林宗龙, 李青. 神经干细胞巢成分及调节机制的研究进展[J]. 中华细胞与干细胞杂志(电子版), 2019, 09(06): 369-374.
[5] 徐如祥, 邱文乔. 生物组装类脑生态位促进神经再生修复展望[J]. 中华神经创伤外科电子杂志, 2022, 08(01): 1-5.
[6] 王洋洋, 高谋, 徐如祥. 过敏毒素、小胶质以及神经干细胞在神经炎症和神经再生中的作用[J]. 中华神经创伤外科电子杂志, 2021, 07(04): 193-198.
[7] 喻勇, 杨华, 徐卡娅. 胎儿脊柱裂的致病因素与治疗的研究进展[J]. 中华神经创伤外科电子杂志, 2021, 07(03): 187-190.
[8] 梁雨婷, 胡悦, 赵安琪, 王沐榕, 孙建, 李力卓. 雌激素作用下神经干细胞移植治疗创伤性脑损伤的研究进展[J]. 中华神经创伤外科电子杂志, 2020, 06(06): 373-376.
[9] 高谋, 徐如祥, 王文佳, 董勤, 丁柏匀, 姚慧, 杨志军. 颅脑创伤动物血清预处理诱导型神经干细胞移植对补体活化的影响[J]. 中华神经创伤外科电子杂志, 2019, 05(04): 227-232.
[10] 高谋, 徐如祥, 董勤, 郭莉丽. CR2-Crry预处理诱导神经干细胞在颅脑损伤中发挥神经保护作用[J]. 中华脑科疾病与康复杂志(电子版), 2021, 11(04): 215-220.
[11] 徐如祥, 杨超, 陈强, 张洪钿. 间充质干细胞治疗阿尔茨海默病的现状与展望[J]. 中华脑科疾病与康复杂志(电子版), 2020, 10(01): 1-5.
[12] 周庆忠, 冯晓兰, 何萍, 张戈, 赵茂, 白永恒, 冯大雄. 封闭Notch信号影响神经干细胞分化的体外研究[J]. 中华临床医师杂志(电子版), 2022, 16(06): 579-587.
[13] 王天仁, 张巧霞, 石瑶, 汤雯珺, 马捷. 神经干细胞在精神分裂症患者治疗中的研究进展[J]. 中华临床医师杂志(电子版), 2020, 14(12): 1002-1008.
[14] 殷秀梅, 杨丽红, 姜涛, 杜元灏. 基于神经干细胞探讨巢蛋白在缺血性脑卒中中的作用机制及针刺效应[J]. 中华针灸电子杂志, 2023, 12(03): 111-116.
[15] 马晓瑭, 王艳, 李素青, 刘金花, 石雨萌, 潘群文. 富含miR-132-3p的神经干细胞释放的外泌体激活MEK1/2/-ERK1/2通路改善缺氧无糖诱导的脑微血管内皮细胞损伤[J]. 中华脑血管病杂志(电子版), 2022, 16(03): 172-181.
阅读次数
全文


摘要