Microstate analysis of prolonged disorders of consciousness based on resting-state electroencephalogram

doi:10.3877/cma.j.issn.2095-123X.2025.04.007

Abstract

Abstract:

Objective

To explore the characteristic electrophysiological markers of patients with prolonged disorders of consciousness (pDOC) by analyzing the resting-state electroencephalogram (EEG) microstate features of patients with pDOC.

Methods

Twenty three patients with pDOC admitted to the Neurosurgery Department of Lu'an People's Hospital Affiliated to Anhui Medical University from January 2021 to March 2024 were selected as the pDOC group, and 17 healthy subjects who underwent physical examination in our hospital were collected as the healthy group at the same time. Video EEG data of resting-state were collected. Microstate clustering was performed using the EEGLAB toolkit based on the Matlab mathematical calculation platform, and statistical analysis was conducted on micro-state indicators (average duration, occurrence frequency, time coverage) between two groups.

Results

The microstate topographic maps A, C and D of the two groups were similar, while the topographic maps of microstate B were significantly different. The time coverage rate of microstate B in the pDOC group (0.37±0.14) was higher than that in the healthy group (0.29±0.10), and the frequency of microstate D (2.96±0.75) and time coverage rate [0.27 (0.23, 0.42)] were lower than those in the healthy group [3.36±0.42, 0.35 (0.30, 0.39)], with statistically significant differences (P<0.05).

Conclusions

The microstate B of pDOC patients has a marked left-right distribution, which may serve as an electrophysiological marker for future assessment of pDOC and degree of consciousness impairment.

Key words: Prolonged disorders of consciousness, Electroencephalogram, Microstate analysis

Guangrui Zhao, Yuzhang Wu, Shaoya Yin, Guanggui Chen. Microstate analysis of prolonged disorders of consciousness based on resting-state electroencephalogram[J]. Chinese Journal of Brain Diseases and Rehabilitation(Electronic Edition), 2025, 15(04): 232-237.

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References 24

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序号	性别	年龄（岁）	病因	CRS-R^a	随访CRS-R
1	女	73	脑出血	VS	MCS-
2	男	49	脑出血	MCS-	MCS-
3	男	63	脑出血	VS	VS
4	男	43	脑出血	MCS-	MCS+
5	女	62	颅脑外伤	VS	VS
6	女	73	脑出血	VS	MCS+
7	男	67	颅脑外伤	VS	MCS-
8	男	70	脑出血	MCS-	MCS-
9	男	20	颅脑外伤	VS	MCS+
10	男	65	脑出血	MCS-	MCS-
11	女	71	脑出血	VS	VS
12	男	67	脑出血	MCS-	MCS-
13	男	67	脑出血	VS	VS
14	女	79	脑出血	VS	VS
15	女	79	脑出血	VS	VS
16	男	70	颅脑外伤	MCS-	MCS-
17	女	57	脑出血	VS	MCS-
18	男	74	脑出血	MCS-	MCS+
19	男	36	颅脑外伤	VS	eMCS
20	女	74	脑出血	MCS+	MCS+
21	男	77	颅脑外伤	VS	VS
22	男	70	脑出血	MCS-	MCS-
23	女	56	脑出血	MCS-	eMCS

序号	性别	年龄（岁）	病因	CRS-R^a	随访CRS-R
1	女	73	脑出血	VS	MCS-
2	男	49	脑出血	MCS-	MCS-
3	男	63	脑出血	VS	VS
4	男	43	脑出血	MCS-	MCS+
5	女	62	颅脑外伤	VS	VS
6	女	73	脑出血	VS	MCS+
7	男	67	颅脑外伤	VS	MCS-
8	男	70	脑出血	MCS-	MCS-
9	男	20	颅脑外伤	VS	MCS+
10	男	65	脑出血	MCS-	MCS-
11	女	71	脑出血	VS	VS
12	男	67	脑出血	MCS-	MCS-
13	男	67	脑出血	VS	VS
14	女	79	脑出血	VS	VS
15	女	79	脑出血	VS	VS
16	男	70	颅脑外伤	MCS-	MCS-
17	女	57	脑出血	VS	MCS-
18	男	74	脑出血	MCS-	MCS+
19	男	36	颅脑外伤	VS	eMCS
20	女	74	脑出血	MCS+	MCS+
21	男	77	颅脑外伤	VS	VS
22	男	70	脑出血	MCS-	MCS-
23	女	56	脑出血	MCS-	eMCS

项目		pDOC组（n=23）	健康组（n=17）	t/Z值	P值
微状态A	出现频率	0.27（0.17，0.40）	0.25（0.13，0.52）	-0.114	0.921
	持续时间（ms）	52.09（44.55，56.47）	56.01（47.86，67.93）	-1.370	0.177
	时间覆盖率	0.015（0.007，0.028）	0.015（0.006，0.038）	-0.485	0.641
微状态B	出现频率	3.07（2.63，3.75）	2.82（2.73，3.14）	-1.710	0.251
	持续时间（ms）	119.18（84.98，141.61）	95.80（77.98，124.06）	-1.456	0.151
	时间覆盖率	0.37±0.14	0.29±0.10	-2.084	0.037
微状态C	出现频率	3.02±0.61	3.21±0.65	-0.885	0.388
	持续时间（ms）	96.00（82.13，122.26）	99.29（90.75，104.38）	-0.542	0.601
	时间覆盖率	0.29（0.26，0.34）	0.34（0.26，0.37）	-1.313	0.196
微状态D	出现频率	2.96±0.75	3.36±0.42	-2.141	0.032
	持续时间（ms）	102.51（81.92，116.99）	97.24（89.12，115.82）	-0.314	0.767
	时间覆盖率	0.27（0.23，0.42）	0.35（0.30，0.39）	-1.999	0.046

项目		pDOC组（n=23）	健康组（n=17）	t/Z值	P值
微状态A	出现频率	0.27（0.17，0.40）	0.25（0.13，0.52）	-0.114	0.921
	持续时间（ms）	52.09（44.55，56.47）	56.01（47.86，67.93）	-1.370	0.177
	时间覆盖率	0.015（0.007，0.028）	0.015（0.006，0.038）	-0.485	0.641
微状态B	出现频率	3.07（2.63，3.75）	2.82（2.73，3.14）	-1.710	0.251
	持续时间（ms）	119.18（84.98，141.61）	95.80（77.98，124.06）	-1.456	0.151
	时间覆盖率	0.37±0.14	0.29±0.10	-2.084	0.037
微状态C	出现频率	3.02±0.61	3.21±0.65	-0.885	0.388
	持续时间（ms）	96.00（82.13，122.26）	99.29（90.75，104.38）	-0.542	0.601
	时间覆盖率	0.29（0.26，0.34）	0.34（0.26，0.37）	-1.313	0.196
微状态D	出现频率	2.96±0.75	3.36±0.42	-2.141	0.032
	持续时间（ms）	102.51（81.92，116.99）	97.24（89.12，115.82）	-0.314	0.767
	时间覆盖率	0.27（0.23，0.42）	0.35（0.30，0.39）	-1.999	0.046

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